In Nintendo’s massive info dump for Super Smash Bros. Ultimate, it was revealed that players will be able to KO assist trophies to earn points during battles. Not only that, but over 50 different assist trophies will be available from the start, including the likes of the Squid Sisters from Splatoon and even Bomberman!
Source: Destructoid Assist trophies can be knocked out in Smash Bros. Ultimate