Spider-Man: Into the Spider-Verse found success by simultaneously being a love letter to and a fresh new take on Spider-Man’s mythology. Although virtually every character featured is based on somebody from the comics, the story and world presented in the film isn’t a precise adaptation of any pre-existing interpretation. This approach gave way to the film’s boldest deviation from the canon: Olivia Octavius, the new Doctor Octopus. But the film’s version of the perennial Spider-Man nemesis goes beyond being only a genderflip; Doctor Octopus has been reinvented here as a modernized, rational character who is obsessed purely with her scientific achievements, refocusing the character on being a mad scientist as opposed to the catch-all supervillain with vague definition that he has been for half a century in the comics.
Source: IGN.com How Spider-Vers Gave Us a Doc Ock for Modern Times